TLR2 and PspA synergistically stimulate PD-L1 up regulation.

<p>For Panel A, mouse bone marrow derived DCs were stimulated with either 15 μg/ml PspA, 1 μg/ml Pam₃Csk₄, 0.2 μg/ml LPS or 1 μg/ml Immiquimod for 24h and surface expression of PD-L1 was monitored by flow cytometry. Dotted lines represent unstimulated controls while thin lines represent stimulations with PspA. Bold lines represent stimulations with indicated agonists to different TLRs. Panel B, DCs were stimulated with 15 μg/ml PspA in the presence or absence of ligands to different TLRs for 24h and PD-L1 expression was monitored by flow cytometry. Thin lines represent stimulations with PspA alone. Bold lines represent costimulations with PspA and indicated agonists to different TLRs. Data from one of three independent experiments are shown. In Panel C, PD-L1 expression is represented as bars indicating fold change in Relative Mean Fluorescence Intensity (MFI) for various groups. Bars represent mean ± SD of three independent experiments. ‘ns’ represents non-significant differences between compared groups.