Anti-PD-1 and anti-PD-L1 synergises with Tcm-phenotype cell transfer to a tolerogenic environment.

<p>A) B16.mOVA cells were analysed for expression of PD-1 and PD-1 ligands. Histogram plots show specific antibody (solid line) and isotype control (dashed line) stained cells. Data are from a single analysis representative of 3 separate analyses. B) B16.mOVA cells (10⁵) were injected s.c. to 11c.OVA mice. Mice were left untreated (●) or 3 days later OT-I Tcm-phenotype cells (5 x 10⁶) transferred i.v. OT-I recipients were injected on the day of OT-I transfer and every subsequent 3 days with isotype control (◯), αPD-1 (▲) or αPD-L1 (▽) mAb. C-E) B16.mOVA cells (10⁵, C; 0.5 x 10⁵, D; 0.25 x 10⁵, E) were injected s.c. to C57BL/6 (C) or 11c.OVA mice (D, E). Mice were left untreated (●) or 3 days later OT-I Tcm-phenotype cells (5 x 10⁶) transferred i.v. OT-I recipients were injected on the day of OT-I transfer and every subsequent 3 days with isotype control (◯), αPD-1 (▲) or αPD-L1 (▽) mAb. Data show survival curves (left) or cumulative mean tumour area ± SEM (right).