miRNA abundances in miRDeep2 and sRNAbench.

<p>A: Proportion of reads mapped to different small RNAs. Values are the average percentage over all samples. Reads matching tRNA dominate. The unmapped percentage is likely due to mismatches in the reads or unannotated ncRNAs. B: Variation of known miRNA discovered with increasing random reads for both methods used in this study. sRNAbench is more sensitive to low abundance genes and produces more hits at a given file size. miRDeep2 was run with no score cut-off. Discovery tails off at 4 million reads in both methods. The inset plot shows the mean abundance of each newly discovered set, illustrating that only low abundance miRNAs are being added after ~3 million mark. C: Overlap between the top 80 miRDeep and sRNAbench results for known miRNAs shows almost identical results. D: Correlation between total read counts determined by both methods for the overlapping miRNAs.