ESMVs contain miRNAs.

<p>The relative abundance of several miRNAs in ESMVs compared with ESCs was determined by real time quantitative RT-PCR. The miRNAs tested include <i>miR-16</i> (lane 1), <i>miR-21</i> (lane 2), <i>miR-22</i> (lane 3), <i>miR-290</i> (lane 4), <i>miR-291-3p</i> (lane 5), <i>miR-292-3p</i> (lane 6), <i>miR-294</i> (lane 7), <i>miR-295</i> (lane 8), and the small nuclear RNA, <i>RNU6b</i> (lane 9). (A) Box plots of relative abundance in ESMVs compared with ESCs (n = 9). The boxed area represents the mean±quartile and the whiskers extend out to the minimum and maximum values. Bootstrap ANOVA was performed and a significant difference was detected between all groups (<i>p</i> = 0.008). (B) The 95^th percentile confidence interval for each miRNA was determined and plotted on a bar graph. Non-overlapping groups are significantly different from each other. RNU6b is significantly less abundant than all miRNAs tested except <i>for miR-22, miR-290,</i> and <i>miR-291.</i> The majority of miRNAs tested do not differ significantly from one another except for <i>miR-295</i>, which is significantly more abundant than <i>miR-290</i> and <i>miR-291</i>.