MAVS50 inhibits MAVS70-dependent IFN induction.

<p>(A and B) 293T cells were transfected with an IFN-β (A) or NF-κB reporter cocktail (B), a plasmid containing MAVS70 and increasing amount of a plasmid containing MAVS50. The promoter activity of IFN-β and NF-κB was determined by luciferase assay at 30 hours post-transfection. <i>**p<0</i>.<i>01</i>; <i>***p<0</i>.<i>005</i>. (C and D) 293T cells were infected with control (CTL) lentivirus or lentivirus containing MAVS50. Whole cell lysates were analyzed with indicated antibodies (C). Stable 293T cells were infected with SeV (100 HA unit/ml) for 8 hours and RNA was extracted. cDNA was prepared and analyzed by real-time PCR with primers specific for <i>IFNβ</i> and <i>ISG56</i> (D). (E and F) 293T cells were transfected with vector or plasmids containing MAVS wild-type (WT), MAVS70 or MAVS50. At 24 hours post-transfection, cells were infected with VSV-GFP (MOI = 0.01). Cells were photographed at 24 hours post-infection (E) and VSV in the supernatant was determined by plaque assay (F).