Characterization of retinal electrophysiology under scotopic conditions.

<p>Representative scotopic ERG traces for VAChT^{Six3-Cre-flox/flox} and VAChT^flox/flox littermate control mice at (A) 5 and (B) 12 months of age at eleven light stimuli of increasing strength. ERG waveforms assessed in response to 11 light stimuli of increasing luminance (0.001 to 25 cd˖s/m²). Waveforms are arranged from lowest stimulus luminance (top) to highest (bottom). Amplitudes of the a-wave (squares) and b-wave (circles) are reduced in VAChT^{Six3-Cre-flox/flox} retinas relative to littermate controls, whereas implicit times of the a- and b-waves are unaffected by the loss of <i>VAChT</i>.; the stimulus onset is indicated by a sideways cross mark.