IL-25 and IL-33 are not required for airway hyperresponsiveness after the last intranasal OVA challenge in mice sensitized epicutaneously (EC) with OVA.

<p>Twenty-four hours after the last inhalation of OVA or PBS, mice sensitized EC with OVA were monitored for pulmonary resistance in response to methacholine. (A) Wild-type (WT) (PBS, n = 7; OVA, n = 7) and IL-25^-/- mice (PBS, n = 7; OVA, n = 7). (B) WT (PBS, n = 5; OVA, n = 8) and IL-33^-/- mice (PBS, n = 5; OVA, n = 12). (C, D) The levels of OVA-specific IgE in sera from mice after intranasal challenge were determined by ELISA. Data show the mean + SEM. *<i>P</i> < 0.05 and **<i>P</i> < 0.01 vs. the corresponding values for PBS-treated mice (A, B). NS (not significant) vs. the indicated group (WT vs. IL-25^-/-, or WT vs. IL-33^-/-)(C, D).