Airway hyperresponsiveness in OVA-sensitised and challenged mice treated with LPS or poly (I:C).

<p>All animals were sensitised i.p. with OVA/Al (OH)₃ and subsequently challenged i.n. with PBS or OVA (3 days) and PBS, LPS or Poly (I:C) (4 days). 24 hrs after the final challenge, changes in lung resistance (R_L) in response to increasing doses of methacholine (MCh) was measured using the flexiVent animal respirator. Data is represented as mean resistance (R_L) ± SEM. (A) Animals were treated with PBS or LPS for 4 days following OVA or PBS treatment. (B) Animals were treated with PBS or Poly (I:C) for 4 days following OVA or PBS treatment. ¤ p<0.05 comparing EC₅₀ values between OVA-PBS and OVA-LPS or OVA-PBS and OVA-Poly (I:C) using one-way ANOVA followed by a Tukey multiple-comparison post-test. ****p<0.0001 comparing R_Lmax of OVA and PBS challenged groups (OVA-PBS vs. PBS-PBS; OVA-LPS vs. PBS-LPS; OVA-Poly (I:C) vs. PBS-Poly (I:C)) using one-way ANOVA followed by a Tukey multiple-comparison post-test. n = 13–16 animals per group.