IL-1α levels in the supernatant of epithelial cell cultures.

<p>IL-1α was measured in supernatants of rat oral mucosal epithelial cell cultures via ELISA. The amount of supernatant assessed on day 8 or 9 was adjusted based on the number of cells for each condition. Calpeptin, <i>n</i> = 3; calpain inhibitor III, <i>n</i> = 3; calpastatin, <i>n</i> = 2; and B27-WT, <i>n</i> = 2. White blocks, controls; blue blocks, inhibitors. The error bars indicate the SD. * <i>P</i> < 0.05 using Student's <i>t</i>-test.