TβH and PHM mutants show similar NMJ phenotypes to <i>nemy</i>^26.2 and <i>nemy</i>⁴⁵ mutants.

<p><b>A</b>. Example traces showing stimulus evoked EJPs and spontaneous mEJPs. <b>B-C.</b> mEJP amplitude (B) and Quantal Content (C) in TβH and PHM mutants and their respective controls. TβH M18 imprecise excision mutants were compared to TβH M6 precise excision controls. PHM^P07623/+ heterozygous mutants (PHM/+) were compared to CS wildtype flies. TβH mutants showed an increase in mEJP amplitude and decrease in Quantal content, while PHM/+ mutants showed an increase in Quantal content, but no change in mEJP amplitude. <b>D-E.</b> Paired pulse plasticity in TβH (D) and PHM (E) mutants and the appropriate controls. Paired pulse plasticity in TβH M18 mutants was not significantly different to that in TβH M6 controls. Paired pulse plasticity in PHM/+ mutants was reduced compared to CS controls. <b>F.</b> Quantal content in control (<i>w</i>) and <i>nemy</i> mutants maintained on regular food (Black bars), or on food supplemented with 10 mg/ml Octopamine (grey bars). On regular food, <i>nemy</i>^26.2 mutants showed a decrease in Quantal content while <i>nemy</i>⁴⁵ mutants showed an increase in Quantal content. Transferring larvae to octopamine supplemented food had no effect on the Quantal content of either control or <i>nemy</i>⁴⁵ animals, but significantly increased the Quantal content of <i>nemy</i>^26.2 mutants. <b>G.</b> Paired pulse plasticity in control and <i>nemy</i> mutants maintained on regular food (no drug) or food supplemented with 10 mg/ml octopamine (octopamine). The paired pulse ratio was not affected in either control (Black symbols) or <i>nemy</i>⁴⁵ mutants (red symbols) on an octopamine supplemented diet (compare open symbols to closed symbols). However, the paired pulse ratio was reduced to control levels in <i>nemy</i>^26.2 mutants (blue symbols) on an octopamine supplemented diet (compare solid inverted triangles in <i>nemy</i>^26.2 mutants on regular food with open triangles of <i>nemy</i>^26.2 mutants on octopamine supplemented food). * indicates P < 0.05 relative to the genetic control. ** indicates P < 0.01 relative to the genetic control. *** indicates P < 0.001 relative to the genetic control. # indicates P < 0.05 relative to the pharmacological control (regular food). Statistical differences were determined by ANOVA followed by multiple comparisons using the Student-Newman-Keuls method.