Additional file 6: Figure S5. of Functional role of dimerization and CP190 interacting domains of CTCF protein in Drosophila melanogaster

Histograms showing dCTCF occupancy in chromatin isolated from mid-late pupa at sequences corresponding to the BX-C insulators Fab-3, Fab-4, Mcp, Fab-6, Fab-8, the Abd-D promoter, and several other dCTCF insulators (9A1, 21E2, 24C4, 27B2, and 57B4R). Chromatin was prepared from 2–3 day old wild type (WT; y 1 w 1118 ) and homozyogous GE24184 (GE/GE) mutant pupae. After fixation and processing, the isolated chromatin was incubated with antibodies directed against the C-terminal region of dCTCF. Sequences from tub, rpl32, and 62D regions were used as negative controls for dCTCF binding. The axis shows the scale for dCTCF enrichment. Error bars show standard deviations of quadruplicate PCR measurements in two biological replicates. The results are presented as a percentage of input DNA. (TIFF 2160 kb