Comparison of phosphorylated myosin light chain immunofluorescence images obtained using different fixative solutions.

<p>Whole-tissue immunofluorescence of pMLC (magenta) was performed on the embryonic epididymis (upper), kidney (middle), and lung (bottom) tissues with a Hoechst dye (cyan) counter stain. Samples were chemically fixed with either 4% paraformaldehyde solution (left) or 2% trichloroacetic acid solution. Epididymis was dissected at E16.5, while the kidney and lung were dissected at E13.5. Scale bar: 50 μm. In the small windows of Fig <b>2A</b> and <b>A′</b>, only pMLC staining images were visualized, and the scale bar represents 20 μm. For the images of immunolabeled pMLC, a maximum intensity projection was applied over 33 μm with 1-μm intervals for the three organs. The images containing Hoechst dye staining are single section views.