Labelling of tetracysteine-tagged hCD36 and immunofluorescence with anti-myc antibody.

Shown are plain HL-1, cells stably overexpressing wildtype of hCD36, and mutant-2, as indicated. Cells were fixed and stained with FlAsH-EDT2 (shown in green). Thereafter hCD36 was immune stained using anti-myc antibody detected by Texas red-labelled secondary antibody (shown in red). Cells were stained with DAPI for visualisation of nuclei (shown in blue). Fluorescent staining was assessed by confocal imaging. Data are representative of three experiments. Images have been adjusted for brightness and contrast for presentation. Scale bars correspond to 10 μm.