Expression of stemness, pluripotency or differentiation related genes in TG1 and OB1 cells under different <i>in vitro</i> culture conditions.

<p>(A-B) Histograms representing mRNA expression levels of stemness, pluripotency or differentiation associated genes included in the TaqMan Human Stem Cell Pluripotency Array from Life Technologies in proliferating (P) and quiescent (Q) (9 days without medium renewal) TG1 (A) and OB1 (B) GSCs. Results were normalized to the 18S rRNA levels and expressed as ΔΔCt taking proliferating TG1 (A) and OB1 (B) GSCs as calibrator samples. Data are from three independent experiments, each performed in duplicate. (C-D) Histograms representing mRNA expression levels of Nanog, GBX2 and IFITM1 genes in proliferating (P) and quiescent (Q) TG1 (C) and OB1 (D) GSCs obtained after 9 days without medium renewal or quiescent cells after 9 days without medium renewal reintroduced in proliferating culture medium for 1–4 days (Q+1-Q+4). Results are expressed as in A-B. Data are from two independent experiments, each performed in duplicate.