Expression of stemness markers.
- Amina Bouacida (121667)
- Philippe Rosset (121669)
- Valérie Trichet (121670)
- Fabien Guilloton (121672)
- Nicolas Espagnolle (121674)
- Thomas Cordonier (121676)
- Dominique Heymann (121679)
- Pierre Layrolle (121682)
- Luc Sensébé (121684)
- Frédéric Deschaseaux (121687)
Publication date
November 2012
DOIAbstract
<p>Quantitative RT-PCR (<b>A</b>) and western blot (<b>B</b>) analysis of the mRNA and protein expression, respectively, of stemness specific markers OCT4A, SOX2 and quantitative RT-PCR analysis of NANOG mRNA expression. Data are mean±SD from 3 experiments. *p<0.01; **p<0.001 compared with PPs. Protein extracts of cells from 3 different BM cultures (BM1, BM2, BM3) of PPs vs MSCs were tested. β-actin was a positive internal control. ND: not determined. MEL1: protein extract of human embryonic stem cell line MEL1 as positive control.</p
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