Additional file 4: Figure S3. of Reversing chromatin accessibility differences that distinguish homologous mitotic metaphase chromosomes

Quantification of inter-homolog probefluorescence intensities following chromosome decondensation with ICRF-193 in independent cell lines. (A–F) Box plots show normalized integrated intensity ratios (y axis) following scFISH for six distinct genomic regions within chromosomes 1q43 (RGS7), 9q34.3 (CACNA1B), 15q13.1 (HERC2), 17p12 (ADORA2B, PMP22:IVS3), and 22q13.33 (ACR) in untreated (−) and treated (+) cells (x axis). GVF measurements in cells hybridized with single copy probes detecting DA from within RGS7, HERC2, PMP22:IVS3, and ACR are indicated from cell line GM10958. Measurements of normalized inter-homolog intensities for CACNA1B and ADORA2B are indicated from cell line GM06326. The same genomic regions were hybridized in opposite cell lines and inter-homolog differences quantified as shown in Fig. 3. Probes detecting DA exhibited larger differences in inter-homolog DNA probe fluorescence (red box plots: median intensity ratios: from 0.68 to 1, n = 125 cells). ICRF-193 treated chromosomes exhibited smaller differences in DNA probe fluorescence (black box plots: median intensity ratios from 0.15-0.39, n = 118 cells) (p < 0.05; two tail t-test), suggesting retrieval of the less accessible chromosome target, except in the case of HERC2, in which DA was not completely reversed. In instances where the median is coincident with the upper quartile, it is emphasized by a thick line to show distinction from the median in the corresponding category