Additional file 4: Figure S3. of Enhanced metastatic capacity of breast cancer cells after interaction and hybrid formation with mesenchymal stroma/stem cells (MSC)

Cell cycle analysis of MDA-hyb1 and MDA-hyb2 cells. Cell cycle analysis was performed by DNA labeling and subsequent FACS measurements in steady state MSCGFP and MDA-MB-231cherry cells as compared to MDA-hyb1 and MDA-hyb2 cells. The cell cycle shift of MDA-MB-231cherry, MDA-hyb1, and MDA-hyb2 cells towards increased fluorescence intensities as compared to MSCGFPdemonstrated an increased amount of DNA and accordingly, aneuploidy in these three cell populations in contrast to a normal diploid set of chromosomes in MSCGFP. Quantification of cell cycle phases was performed using FlowJo software. (PDF 192 kb