High glucose inhibits induction of <i>Abca1</i> in RAW macrophages.

<p>RAW WT macrophages were treated for four hours with 5 μM T + 1 μM 9cisRA (T+9) or DMSO vehicle control and steady state RNA (A) and nascent RNA (B) transcripts of <i>Abca1</i> were profiled using qRT-PCR. <i>Cyclophilin A</i> was used for normalization. Macrophages were cultured overnight in 1% FBS prior to treatment. Panel A represents an average of five independent experiments and panel B represents an average of three independent experiments. Error bars indicate the SEM. Significance is determined using the two-tailed Student's t-test (*, P < 0.05, **, P<0.01, ***, P<0.001).