Conditioned FBS-containing hCLCA1 medium shows time-dependent effect.

<p>Macrophages were activated with 1.0 μg/mL LPS, 1.0 mg/mL FBS-containing wild-type hCLCA1, E157Q mutant hCLCA1 or eGFP medium. Time-dependent (1, 2, 4, 6, 12, 24 h) cytokine mRNA expression by macrophages was quantified through RT-qPCR. The fold difference at each condition was compared against eGFP (the control) of the same condition. Results were the means of 4 samples ± SEM. Each sample was a result of an individual transfection paired with an eGFP transfection. Significant fold differences from the corresponding control values (eGFP of the same concentration) are indicated by * (<i>p</i> < 0.05), ** (<i>p</i> < 0.005) or *** (<i>p</i> < 0.001).