M2 receptor expression is decreased in presymptomatic stages in the SOD1 ventral spinal cord.

<p><b>(A)</b> Representative protein levels of the M2 muscarinic receptor detected by western blot in wild type (WT) and SOD1 (SOD) mice at different postnatal (P) stages. All blots were reprobed for β-actin as a loading control. The molecular weight in kDa is shown on the left side of the picture. (<b>B</b>) Mean M2 receptor/β-actin expression ratio as a function of animal age in WT (black bars) and SOD1 (white bars) mice. At least four WT and four SOD1 mice were tested in each group. Comparisons between groups were made with two-way ANOVA with Sidak’s multiple comparison tests. Asterisks indicate positive significance (p<0.05).