VEGF and VEGF receptor protein levels in the cortex and spinal cord of pre-symptomatic and symptomatic SOD1^G93A mice.

(A-D) Data from cortical samples; (E-H) data from spinal cord samples. VEGFA (A, E), VEGFB (B, F), VEGFR-1 (C, G) or VEGFR-2 (D, H) protein levels, determined by ELISA, in samples from pre-symptomatic (4–6 weeks old) or symptomatic (12–14 weeks old) SOD1G93A and age-matched WT mice, as indicated below each data set. The Y-axis represents the ligand or receptor levels expressed in pg/mg of total protein in each condition. Data are expressed as mean ± SEM (n = 3–7 animals); *p < 0.05, **p ≤ 0.01, ***p ≤ 0.001, two-way ANOVA with Holm-Sidak correction for multiple comparisons.