Effect of the overexpression of <i>SlANT1</i> and <i>SlAN2</i> on other genes of the anthocyanin pathway.

<p>Quantitative analysis of transcript levels of <i>SlAN2</i>, <i>SlANT1</i>, <i>SlAN1</i>, <i>SlAN11</i>, <i>SlJAF13</i> and <i>SlDFR</i> in leaves and peel from green fruits of the 35S:<i>SlANT1</i> and 35S:<i>SlAN2</i> lines in comparison with control Ailsa Craig plants (A). Expression levels are shown as relative units, with the value of AC leaves set to one. A sample composed of two biological replicates was analyzed for each plant tissue and data are means of two technical replicates ± SD. 35S:<i>SlANT1</i> line T9002 and 35S:<i>SlAN2</i> line R9009 were chosen for the qPCR analysis. Transient transformation experiment in Arabidopsis mesophyll protoplasts showing that both SlAN2 and SlANT1 activated the <i>SlDFR</i> promoter (B). Protoplasts were transfected with the reporter plasmid containing the <i>SlDFR</i> promoter driving firefly luciferase (PpLuc) gene alone (first histogram) or in combination with the effector plasmid containing either the full length <i>SlAN2</i> or <i>SlANT1</i> coding sequence (second or third histograms, respectively). A 35S:Renilla-luciferase (RrLuc) plasmid was used as an internal control. Data are expressed as Relative Luc Activity (RLU) (PpLuc/RrLuc) and are means of eight biological replicates ± SE.