Transient expression of putative regulators of anthocyanin synthesis in leaves of <i>Nicotiana tabacum</i>.

<p>(A) Infiltrations of single genes. (B) The effect of <i>PaHLH3</i> and <i>PaHLH33</i>. (C) Infiltrations of <i>PaMYB10</i>.<i>1–1</i>. (D) Infiltrations of <i>PaMYB10</i>.<i>1–2</i>. (E) Infiltrations of <i>PaMYB10</i>.<i>1–3</i>. (E) A comparison between infiltrations of <i>PaMYB10</i>.<i>1–3</i> and <i>PaMYB10</i>.<i>1-3k</i>. Infiltrated control cDNAs: PAP1, <i>Arabidopsis thaliana PAP1</i> (MYB75); GFP, <i>Aequorea victoria GFP</i>. Tested <i>Prunus avium</i> genes are isolated from following cultivars: <i>PaWD40</i>, <i>PaHLH33</i>, <i>MYB10</i>.<i>1–1</i>, <i>MYB10</i>.<i>1–3</i> from ‘Irema BS’; <i>PaHLH3</i> from ‘Kitayanka’; <i>MYB10</i>.<i>1–3</i> from ‘Regina’. <i>MYB10</i>.<i>1-1k</i>—cDNA from ‘Kitayanka’ fruits; <i>MYB10</i>.<i>1-3k</i>—cDNA from infiltrated ‘Irema BS’ gene expressed in <i>N</i>. <i>benthamiana</i> leaves.