MiR-124 downregulates flotillin-2 expression by directly targeting its 3ʹ-UTR in mouse testicular tissue.

<p>(a) Putative duplex formation between miR-214 and the flotillin-2 3ʹ-UTR. The binding site is conserved between several mammalian species (in bold and boxed). (b) Assay constructs, wild-type and mutated target sequences of flotillin-2. (c) MiR-124 downregulated the luciferase activity of the flotillin-2 3ʹ-UTR construct, whereas luciferase activity was not significantly decreased in the target region of the mutated mut 3ʹ-UTR construct *<i>p</i> < 0.05 compared with control (two-tailed). Error bars indicate SD. (d) MiR-124 expression in mouse brain tissue and mouse testicular tissue.