miR-34bc/449 regulates a small cohort of genes in spermatocytes.

<p>(<b>A</b>) Expression scatterplot showing relative average expression of affymetrix probes between control (x-axis) and miR-34bc^−/−;449^−/− (y-axis). Significantly deregulated (p = 0.05) genes with a log₂ fold change of 0.5 (red) are shown. (<b>B</b>) The list of the 13 upregulated genes with predicted miR-34 seed binding sites is shown. Also indicated is the gene function as well as number of miR-34 binding sites. (<b>C</b>) qRT-PCR expression analysis of representative miR-34 family seed-containing deregulated genes identified. Normalized data are plotted as relative fold change in miR-34bc^−/−;449^−/− versus wild type pachytene spermatocytes. Standard error is shown and the asterisk indicates significantly upregulated expression (P<0.05). Other genes identified from the array that change in expression are also presented. The data in all panels are from biological quadruplicates of each genotype. (<b>D</b>) Sylamer enrichment landscape plot for all 876 7 nt words complementary to canonical mouse miRNA seed regions. The y-axis represents the sorted genelist of 21,560 genes from most up-regulated to most down-regulated in the miR-34bc^−/−;449^−/− pachytene spermatocytes. Each 7mer word was tested for significant enrichment across the 3′UTRs of genes in this list. The word corresponding to seed matching miR-34 family (Red) is enriched in the up-regulated genes.