Rheb prevents cardiac hypertrophy and interacts with Bnip3.

<p>Myocardial lysates from Bnip3-TG and wild type (WT) mice were prepared and processed for reciprocal Bnip3 and Rheb immunoprecipitation as described in Methods. Representative western blots showing immunoprecipitation (i.p.) and western blot (WB) antibodies are shown in (A). Quantification of western blot immunoprecipitation is shown in (B) (n = 3; * p < 0.002). (C) Representative plates of neonatal rat cardiac myocytes after transfection with random sequence (RS) or Rheb siRNA and exposure to serum as a hypertrophy stimulus as described in Methods. Top panels GFP; bottom phalloidin (red); arrows indicate hypertrophied myocytes in RS panels and reduced myoplasm area with Rheb-siRNA treatment. (D) Quantification of cell area from ~100 myocytes per group as described in Methods (n = 3; * p < 0.002). (E) Representative western blot shown efficient KD of Rheb by siRNA. All results are mean ± S.E.M.