Mutant <i>rad53 swe1 pds1</i> cells elongate spindles in the presence of DNA damage.

<p>Cells were grown to mid-exponential phase, synchronized in G1 phase with the pheromone alpha-factor, then released into S phase in the presence of 0.033% MMS. Results correspond to cells 240 minutes after the release from G1. (A) Spindle lengths were measured in wild type (WT, strain YGP20), <i>swe1</i> (YGP98), <i>pds1</i> (strain YRP33), <i>rad53 swe1</i> (YGP121), <i>rad53 pds1</i> (strain YGP208), and <i>rad53 swe1 pds1</i> (strain YGP201) cells. Cells were fixed, probed with anti-tubulin antibody, to visualize spindles, and stained with Hoechst 33258, to visualize DNA by fluorescence microscopy. Spindle length in 200 cells for each strain were measured and represented as box-and-whisker plots. (B) Representative cells obtained by double fluorescence with wild type (WT, strain YRP117), <i>swe1</i> (YRP118), <i>pds1</i> (strain YRP159), <i>rad53 swe1</i> (YRP165), <i>rad53 pds1</i> (strain YRP164), and <i>rad53 swe1 pds1</i> (strain YRP144) cells. Spindles (Tub1-GFP) and chromatin (Htb2-mCherry) and were visualized by fluorescence microscopy.