Mitotic phenotype observed upon depletion by siRNA of the selected predicted spindle proteins.

<p>(<b>A</b>) HeLa S3 cells were treated for 48 h with control (GL2) or <i>KIAA0841</i>-and <i>Nup88</i> specific siRNAs, respectively, then fixed and stained with α-Tubulin (green). DNA was visualized using DAPI (blue). Bar = 10 µm. (<b>B</b>) HeLa S3 cells were treated for 48 h with control (<i>GL2</i>) and p59Fyn specific siRNAs, respectively, then fixed and stained with α-Tubulin (green). DNA was visualized using DAPI (blue). Bar = 10 µm. (<b>C–G</b>) Stills of representative movies of H2B-GFP expressing HeLa S3 cells treated with control (<i>GL2</i>), <i>KIAA0841</i>, <i>Nup88</i>, <i>p59Fyn</i> and <i>WD75</i> siRNAs for 36 h before filming. Time points are indicated in h:min.