Effect of CBS silencing on H₂S production in human T24 cells.

<p>(A) Generation of CBSΔT24 cells. Five clones resistant to puromycin were screened for CBS expression using western blotting analysis. Clone F was selected for the further experiments. GAPDH was used as a protein loading control. (B) The H₂S production in CBSΔT24 is markedly lower than in control T24 cells (*p<0.05). Data are calculated as nanomoles per milligram of protein per min and expressed as mean±SE of four different experiments.