Effects of NaHS on H₂S levels and H₂S synthesizing enzyme activities.

<p>(<b>A</b>) The changes of H₂S levels in medium for each treatment group (expressed in μM). (<b>B</b>) CSE activities in HUVECs lysate of each group, presented as μmol/h/g. (<b>C</b>) CSE protein expressions levels as determined using western blot analysis. (<b>D</b>) CSE mRNA expression levels as determined by real-time PCR. (<b>E</b>) CBS protein expressions levels and (<b>F</b>) The CBS mRNA expression tested by real-time PCR. The values in (C)–(F) were normalized against the control values. The data shown are mean ± SEM (n = 6). * <i>p</i><0.05, ** <i>p</i><0.01 vs control. # <i>p</i><0.05 vs vehicle + H₂O₂ group.