FLG2 is detected in eccrine sweat glands and sweat.

<p>A-C: Immunohistochemistry in skin sections. Especially eccrine sweat glands were stained using the antibody directed against FLG2-4 (A, B). C: control section without primary antibody. D: Western Blot analysis of sweat shows an intense staining for FLG2-4 above 70 kDa only in samples pretreated for 1h with 10 mM DTT. A representative experiment out of four is shown. S/C: complete sweat sample, S/P: sweat pellet resuspended in PBS, S/S: sweat supernatant, separated by centrifugation. FLG2: FLG2-4 recombinant fragment. Approximate molecular mass in kDa is indicated on the left.