MntABC is important for efficient proliferation of <i>S</i>. <i>aureus</i> after oxidative stress.

<p>(A) Percentage of wild-type and <i>mntC</i> mutant cells that lost CFSE fluorescence when inoculated into TSB media after recovery from IFN-γ activated murine macrophages with and without DPI treatment. Bacteria were identified by cell size and further differentiated from macrophage debris by co-staining with an anti-<i>S</i>. <i>aureus</i> antibody. Data are median values from triplicate samples with standard deviation. (B) Wild-type and <i>mntC</i> mutant cells were treated with 1 μM methyl viologen (MV) for 1 hour. Growth after treatment was measured at indicated time points for 12 hours post exposure, via an increase in BacTiter-Glo luminescence values. Once bacterial cultures reached stationary phase growth, growth was no longer measured. Data are mean values of triplicated samples with standard deviation.