<p>CI-CV: mitochondrial complexes I-V, ADP: adenosine diphosphate, FCCP: carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone, Protocol<sub>Int</sub>: protocol for intact cells, SUIT: substrate-uncoupler-inhibitor-titration protocol for electron flow through CI, CII and convergent CI+II.</p><p>Reagents used in the different high-resolution respirometry protocols.</p
<p>Oxygen consumption rate (OCR) is measured before and after the addition of inhibitors to derive s...
The mechanism of uncoupling of oxidative phosphorylation by carbonyl cyanide p-(trifluoromethoxy)phe...
A high-resolution oxygraph is a device for measuring cellular oxygen consumption in a closed-chamber...
<p>SUIT1 (Fig 1A) was designed to primarily examine control of OXPHOS capacity. SUIT2 (Fig 1C) was d...
258-272Isolated mitochondria serve as a biological model for analysis of oxidative phosphorylation a...
(A) Maximal complex I (CI) mediated OXPHOS capacity (state 3) respiration was determined in the pres...
<p>A,B. Oxygen consumption was assessed by high-resolution respirometry in digitonin-permeabilized N...
potential; RCI, respiratory control index; TPP, tetraphenylphosphonium; GDP, guanosine diphosphate; ...
<p>Intact EA.hy 926 cells were assayed by high-resolution respirometry in the buffer described in Ma...
Functional studies on isolated mitochondria critically rely on the right choice of respiration buffe...
<p><i>A</i>: Increase in AMP/ATP and ADP/ATP ratios in HepG2 cells. The cells were treated with 0.0...
<p>(A) Protocol with intact cells, (B) SUIT<sub>CI</sub> protocol with permeabilized cells with glut...
Functional studies on isolated mitochondria critically rely on the right choice of respiration buffe...
<p><b>A</b>: SUIT1 protocol with electron flow through CI supported by glutamate+malate. <b>B</b>: S...
Whereas mitochondria are well established as the source of ATP in oxidative phosphorylation (OXPHOS)...
<p>Oxygen consumption rate (OCR) is measured before and after the addition of inhibitors to derive s...
The mechanism of uncoupling of oxidative phosphorylation by carbonyl cyanide p-(trifluoromethoxy)phe...
A high-resolution oxygraph is a device for measuring cellular oxygen consumption in a closed-chamber...
<p>SUIT1 (Fig 1A) was designed to primarily examine control of OXPHOS capacity. SUIT2 (Fig 1C) was d...
258-272Isolated mitochondria serve as a biological model for analysis of oxidative phosphorylation a...
(A) Maximal complex I (CI) mediated OXPHOS capacity (state 3) respiration was determined in the pres...
<p>A,B. Oxygen consumption was assessed by high-resolution respirometry in digitonin-permeabilized N...
potential; RCI, respiratory control index; TPP, tetraphenylphosphonium; GDP, guanosine diphosphate; ...
<p>Intact EA.hy 926 cells were assayed by high-resolution respirometry in the buffer described in Ma...
Functional studies on isolated mitochondria critically rely on the right choice of respiration buffe...
<p><i>A</i>: Increase in AMP/ATP and ADP/ATP ratios in HepG2 cells. The cells were treated with 0.0...
<p>(A) Protocol with intact cells, (B) SUIT<sub>CI</sub> protocol with permeabilized cells with glut...
Functional studies on isolated mitochondria critically rely on the right choice of respiration buffe...
<p><b>A</b>: SUIT1 protocol with electron flow through CI supported by glutamate+malate. <b>B</b>: S...
Whereas mitochondria are well established as the source of ATP in oxidative phosphorylation (OXPHOS)...
<p>Oxygen consumption rate (OCR) is measured before and after the addition of inhibitors to derive s...
The mechanism of uncoupling of oxidative phosphorylation by carbonyl cyanide p-(trifluoromethoxy)phe...
A high-resolution oxygraph is a device for measuring cellular oxygen consumption in a closed-chamber...