<i>KRAS</i> mutant FFPE tissue DNA analysis using multiplex and duplex assays to detect <i>KRAS</i> mutant clones.
- Alexandra Pender (800996)
- Isaac Garcia-Murillas (502434)
- Sareena Rana (94145)
- Rosalind J. Cutts (800997)
- Gavin Kelly (800998)
- Kerry Fenwick (41196)
- Iwanka Kozarewa (179955)
- David Gonzalez de Castro (5651461)
- Jaishree Bhosle (800999)
- Mary O’Brien (801000)
- Nicholas C. Turner (265334)
- Sanjay Popat (18278)
- Julian Downward (49269)
Publication date
September 2015
DOIAbstract
<p>All samples, except for S011, were analysed with multiplexes A, B and C (upper panels) and the <i>KRAS</i> mutation detected was subsequently confirmed with the appropriate duplex assay (lower panels). Mutant DNA droplet populations are highlighted with a red dashed square. Droplet populations caused by cross-reactivity with a <i>KRAS</i> mutant DNA species not present in the multiplex are indicated by a yellow dashed square.</p
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