Rotor-Gene 6000 PCR amplification plots of SensiScreen<sup>®</sup> <i>KRAS</i> exon 2 multiplex assays using serial dilutions of mutated DNA in a wild type background.
- Alice Riva (4164367)
- Michael BØrgesen (4164370)
- Mariann Guldmann-Christensen (4164394)
- Majbritt Hauge Kyneb (4164382)
- Kirsten Voogd (4164391)
- Christina Andersen (3105336)
- Samantha Epistolio (4164376)
- Elisabetta Merlo (4164388)
- Tine Yding Wolff (4164385)
- Stephen Hamilton-Dutoit (69422)
- Jan Lorenzen (4164373)
- Ulf Bech Christensen (4164379)
- Milo Frattini (370150)
Publication date
June 2017
DOIAbstract
<p>50 ng and/or approximately 16,000 copies of DNA was added to each reaction. The threshold was set at 10% of the average fluorescence signal of the reference assay at cycle 45. Legend describes the fraction of cell line DNA and/or mutated copies of KRAS exon 2 templates.</p
Add to ORKG