Electrophoretic mobility shift assay (EMSA) for testing the DNA binding ability of Stl constructs.

<p><b>(A)</b> Result of EMSA with the wild type Stl protein. Note that the DNA band is shifted upwards at 1 μM Stl concentration. <b>(B)</b> EMSA gel result of Stl-AA mutant protein. Note that even at relatively high (ie. 3 μM) concentration of the Stl-AA mutant construct, most DNA still appears at the lower position on the gel, indicating lack of binding to the mutant protein.