Electrophoretic mobility shift assay (EMSA).

<p>We incubated a double-stranded oligonucleotide (dsDNA) with increasing concentrations of peptides containing either the wildtype (²⁸Gly) or mutant (²⁸Glu) sequence of the first AT-hook of the <i>HMGA2</i> protein. With the wildtype sequence a partial shift of the dsDNA band could already be observed at the lowest concentration of peptide corresponding to a molar ratio of peptide to DNA of 1.4. At the highest concentration of the wildtype peptide, the majority of the DNA was in the DNA-protein complex. In contrast, with the mutant peptide the band shift was only observed at higher peptide concentrations and a smaller proportion of DNA was bound.