Muscle-infiltrating CD4⁺ and CD8⁺ T cells isolated from LysMcre;Arg1^F/F mice express enhanced IFN-γ transcript.

<p>(A) Representative flow plots demonstrating the gating strategy to FACS-sort CD19^-CD3⁺CD4⁺CD8^- and CD19^-CD3⁺CD8⁺CD4^- T cells isolated from quadriceps muscles of RRV-infected WT or LysMcre;Arg1^F/F mice at 10 dpi and the post-sort purities. RT-qPCR analysis of (B) IFN-γ, (C) TNF-α, (D) IL-10, and (E) IL-2 expression in FACS-sorted T cells from the spleen of a mock-inoculated mouse (n = 1) or from the quadriceps muscle of RRV-infected WT (n = 3) or LysMcre;Arg1^F/F mice (n = 3) at 10 dpi. Data are normalized to 18S rRNA levels and are expressed as the relative expression (<i>n</i>-fold increase) over expression in spleen T cells from a mock-inoculated mouse. Data are represented as the arithmetic mean ± SEM. Data are representative of two independent experiments. <i>P</i>-values determined by two-tailed, unpaired <i>t</i>-tests with or without Welch’s correction. nd, not detected.