MOESM1 of A particular silent codon exchange in a recombinant gene greatly influences host cell metabolic activity

Additional file 1. Quantitative classification of E. coli BL21(DE3) clones according to respiration behavior adapted from Rahmen et al. [23]. Respiration behavior (oxygen transfer rate, determined using a RAMOS device) as function of time for one example each for a Type A and Type B clone. According to Rahmen et al. [23] integral X represents protein production phase, whereas integral Y represents the subsequent growth phase on glycerol. X/Y > 1.2 categorizes Type A clones, X/Y < 1.2 Type B clones. Calculated X/Y ratios for all investigated clones are given in Table 1. Cultivation conditions: 37 °C, 250 mL flasks, filling volume 10 mL, shaking frequency 350 rpm, shaking diameter 50 mm (in RAMOS)