MOESM2 of A particular silent codon exchange in a recombinant gene greatly influences host cell metabolic activity

Additional file 2. Respiration activity of E. coli BL21(DE3) clones containing amino acid exchanges with synonymous codons grown under inducing conditions. Respiration behavior (oxygen transfer rate) as function of time for clones containing amino acid exchanges with synonymous codons during cultivation in Wilms-MOPS mineral autoinduction medium containing 0.5 g/L glucose, 5 g/L glycerol, 2 g/L lactose. Clones S56P, S167P, and G175F can be categorized into Type A independent from codon, clones K170E into Type B. Clones D91R and G93Y are categorized into Type A (D91R-CGC, G93Y-TAT) or Type B (D91R-CGT, G93Y-TAC) according to the respective synonymous codon. All cultivation experiments were performed at least in duplicates. Cultivation conditions: 37 °C, 250 mL flasks, filling volume 10 mL, shaking frequency 350 rpm, shaking diameter 50 mm (in RAMOS)