FoxM1 and Sox2 are upregulated in GBM cells after irradiation.

<p>(A) Western blot analysis of FoxM1 and Sox2 using the irradiated NS07-448 (left) and 387 (right) GBM cells. Cells were harvested with course of time after irradiation. β-actin was used as a loading control. (B) Representative IF images of NS07-448 GBM cells with or without irradiation using anti-FoxM1 and anti-Sox2 antibodies. Scale bar, 20 μm. (C and D) Western blot analysis of FoxM1 and Sox2 in GBM cells after irradiation. Cytoplasmic (C) and nuclear (N) fractions of protein lysates were prepared to determine FoxM1 and Sox2 changes 6 and 12 hours after irradiation. As loading controls, α-tubulin (cytoplasm) and TBP (nuclear) were used. Quantitation of these protein bands were shown in (D).