Effect of Pu27-HS on SOX2 expression in U937 after 3 days exposure.

<p>(A). Bar graph representing RT-qPCR (C_T values) for <i>SOX2</i> (red bars) compared to <i>c-MYC</i> (gray bars) expression in 4 leukemia cell lines and PBMC from 2 donors. (B) Western blot for SOX2 protein expression in 4 leukemia cell lines and PBMC. Bar graph for the quantification of SOX2 protein normalized to α-tubulin. (C). Evaluation of <i>SOX2</i> expression compared to c-MYC after 72h exposure to 10μM Pu27-HS. Data represent the average of fold change for treated compared to untreated +/- SD of 3 independent experiments (*<i>p</i><0.05). (D). Evaluation of SOX2 protein expression in U937 by Western Blot, representative blot is shown. Quantification of SOX2 normalized to α-Tubulin, bar graph shows the average of intensity of the bands for 2 separate experiments.