RT-qPCR for <i>CBR</i>.1. <i>CBR</i>.2, <i>CYB5</i> and <i>crtR</i> transcript levels in the wild-type and mutant strains.

<p>(A) <i>CBR</i>.1 (B) <i>CBR</i>.2, (C) <i>CYB5</i> and (D) <i>crtR</i> genes. The transcript level of each gene was normalized to actin mRNA. Black and grey bars represent the results using RNA extracted after 36 and 72 h of culture, respectively. Values are the mean ± standard deviation of two technical replicates from three independent cultures (Student’s t-test was performed to compare each mutant to the parental strain. * P <0.05 and ** P <0.01).