Alum in FI-RSV enhances lung viral clearance but leads to severe weight loss.

<p>At 12 weeks post-boost immunization, immune and PBS mock control mice (n = 5 per group) were i.n. challenged with 2x10⁶ PFU RSV A2. Naïve: unimmunized mice without RSV infection, PBS: PBS mock control mice with RSV infection, live RSV: live RSV re-infection mice after RSV infection, FI-RSV-A: FI-RSV-A immune mice after RSV challenge, FI-RSV: FI-RSV immune mice after RSV challenge. (A) Body weight changes in naïve and prior live RSV reinfected mice after RSV challenge. (B) Body weight changes in naïve, FI-RSV-A, and FI-RSV mice after RSV challenge. (C) RSV titers in lungs. After 5 d.p.c., individual lungs were collected and virus titers were determined. Results are presented as mean ± SEM (n = 5). Statistical significances were performed by two- or one-way ANOVA and Tukey’s multiple comparison tests in GraphPad Prism; (A and B) <i>p</i><0.001, compared with naïve control and immunized or RSV reinfection group; (B) ^##<i>p</i><0.01, ^###<i>p</i><0.001 between with FI-RSV-A and FI-RSV.