Over-expression of Ly-GDI decreases IgG immune complex-induced generation of pro-inflammatory mediators in BAL fluids.

<p><b>(A-D)</b> Adeno-GFP or Adeno-Ly-GDI was injected to mice <i>via</i> an airway instillation at a dose of 5 × 10⁸ PFU. 3 days later, the mice were subjected to IgG immune complex-induced lung injury or control treatment (αBSA only). Cell-free BAL fluids were harvested 4 h later, and ELISAs were conducted to measure the TNF-α <b>(A)</b>, IL-6 <b>(B)</b>, MCP-1 <b>(C)</b>, and MIP-1α <b>(D)</b> levels. <b>(E and F)</b> MH-S cells were transiently transfected with indicated genes. 48 h later, the cells were treated with or without 100 μg/ml IgG immune complexes for 4 h. The cell lysates were used to evaluate luciferase expression. Luminometer values were normalized for expression from a co-transfected thymidine kinase reporter gene. These values were also normalized to a relative value of 1 for the control plasmid-transfected cells that did not receive IgG immune complex stimulation. Data were expressed as means ± S. E. M. N ≥ 3 mice for each group. *, ** and *** indicated statistically significant difference- <i>p<0</i>.<i>05</i>, <i>p</i> < 0.01 and <i>p</i> < 0.001, respectively.