MLE is required for full expression of genes in heterochromatic regions.

<p>Expression was measured in <b>A)</b><i>mof</i>^<i>1</i>, <b>B)</b><i>mof</i>^<i>2</i>, <b>C)</b><i>mle</i>^<i>1</i> and <b>D)</b><i>Jil-1</i>^<i>z2</i> males. Controls were heterozygous (<i>mle/+; Jil-1/+</i>) or complemented (<i>mof</i>; [<i>mof</i>^<i>+</i>]) animals. Gene groups are euchromatic autosomal (ch 2 and 3, 6 genes analyzed, excepting <i>mle</i>^<i>1</i> with 8 genes analyzed); X-linked (4 genes); heterochromatic (ch 2 and 3, 10 genes) and 4^th-linked (14 genes). Genes and primers are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140259#pone.0140259.s006" target="_blank">S1 Table</a>. Expression of individual genes is presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140259#pone.0140259.s001" target="_blank">S1 Fig</a>. The normalized Log₂ expression ratio (mutant/control) is plotted (see methods for details). Box plots were generated using R. A Wilcoxon test comparing each gene group with euchromatic autosomal genes was used to determine <i>p-</i>values. Differences significant at a <i>p-</i>value of < 0.05 are marked with asterisks.