Contribution of MMR to the stability of heterochromatic DNA at <i>hml</i> and a Chr VII-L telomeric region.

<p>(<b>A</b>) Outline of heterochromatic <i>HML</i>, <i>hml</i>::<i>URA3</i>, Chr VII-L, and Chr VII-L::<i>URA3</i> regions. (<b>B</b>) Deletion of <i>MSH2</i> in the wild-type strains does not disrupt heterochromatin at the <i>hml</i>::<i>URA3</i> and Chr VII-L::<i>URA3</i> loci. The experiments were carried out as described in <b><a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007074#pgen.1007074.g001" target="_blank">Fig 1B</a></b>. The data are shown as averages ± 1 S.D. (n≥6). (<b>C</b>) Spontaneous mutation rates at the heterochromatic <i>hml</i>::<i>URA3</i> and Chr VII-L::<i>URA3</i> loci in the isogenic wild-type and <i>msh2Δ</i> strains. The wild-type strains were BKDY438 and BKDY541. The mutation rates were measured as described under Materials and Methods. 95% confidence intervals are in parentheses.