MOESM3 of High-level secretion of native recombinant human calreticulin in yeast

Additional file 3. Western blotting analysis of recombinant and native human CRTs. The same samples were analysed by SDS-PAGE (A) and Western blot (B) using monoclonal antibodies against human CRT. M—prestained protein ladders. pFDC and pFDC-CRT—10× concentrated culture media samples from S. cerevisiae transformed with pFDC vector and pFDC-CRT plasmid, respectively. Sc, Pp and Hn—purified CRT from S. cerevisiae, P. pastoris and human placenta, respectively. Crude lysate of S. cerevisiae cells transformed with pFDC vector was used as negative control for Western blot. Bl—blank lanes