MOESM1 of Yeast lysates carrying the nucleoprotein from measles virus vaccine as a novel subunit vaccine platform to deliver Plasmodium circumsporozoite antigen

Additional file 1: Figure S1. Western blot analysis of clarified yeast lysates prepared from Pichia pastoris expressing N-PbCS (A) or N (B) at five different time points. Yeasts were cultured in independently prepared batches of media and lysed according to the standardized protocol. Samples of yeast lysates (non-diluted for N-PbCS and diluted 1/125 for N lysates) were prepared for western blot analysis and stored at −80 °C. Anti-N monoclonal antibody was used to detect N-PbCS and N proteins. Despite the presence of protease inhibitors in the lysis buffer, reproducible patterns of proteolysis were observed in the N-PbCS fusion protein, as previously observed [6]