The effects of amino acids on the final mAb titre, the effect parameter is the change in the final mAb titre as the amino acid concentration changes from its low level to its high level.

<p>* Amino acids in bold with a contribution% greater than 0.1 were chosen for the model in ANOVA.</p><p>Sum of square is the sum of squared deviations from the mean due to the effect of the term. Contribution% determines the individual amino acid contribution to final mAb titre in comparison to others.